Predictive value of CK20 and villin expression in postoperative outcomes and prognosis of colorectal cancer

PENG Weibin; XIAO Huaxu; WANG Mingtao

doi:10.3969/j.issn.1009-0460.2025.09.006

Abstract：Objective To investigate the expression and clinical significance of cytokeratin 20(CK20)and Villin in colorectal cancer tissues.Methods A total of 73 patients with colorectal cancer admitted to the First People's Hospital of Yibin from January 2021 to December 2021 were collected as the research subjects and divided into the good prognosis group(n=46)and the poor prognosis group(n=27).The expressions of CK20 and Villin proteins in tissues were detected by immunohistochemical staining,and the relationship between their expressions and the clinicopathological characteristics of colorectal cancer was analyzed.The receiver operating characteristic curve was used to evaluate the value of both expressions in prognosis assessment.The Cox proportional hazards regression model was used to analyze the factors influencing the prognosis of colorectal cancer.Results The positive expression rates of CK20 and Villin in colorectal cancer tissues were significantly higher than those in adjacent tissues(CK20:69.9%vs.41.1%;Villin:67.1%vs.38.4%).Poor prognosis of patients was related to positive expression of CK20(χ2=5.168,P=0.023),positive expression of Villin(χ2=6.648,P=0.010),degree of differentiation(χ2=6.367,P=0.012),and T stage(χ2=4.528,P=0.033),N stage(χ2=5.013,P=0.025)and tumor size(χ2=7.138,P=0.008)were related.The ROC curve results showed that both positive expressions of CK20 and Villin had good predictive value for the poor prognosis of colorectal cancer,with the areas under the curve(AUC)being 0.838 and 0.823,respectively.The results of multivariate Cox regression showed that positive expression of CK20,positive expression of Villin,N stage,tumor size and degree of differentiation were factors affecting the poor prognosis of patients with colorectal cancer.Conclusion The expression levels of CK20 and Villin can be used as effective indicators for predicting adverse clinical outcomes in patients with colorectal cancer.