Effects of circular RNA_0056618 on the biological behavior of esophageal cancer Eca109 cells

GUO Xiaoyan; CUI Yingxue; CHEN Boting

doi:10.3969/j.issn.1009-0460.2025.07.002

Abstract：Objective To explore the effect of circular RNA_0056618(circ_0056618)on the proliferation,migration and invasion of esophageal cancer Eca109 cells.Methods Levels of circ_0056618 and microRNA-1281(miR-1281)in cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR.The small interfering RNA sequence of circ_0056618(si-circ_0056618),miR-1281 mimics and their negative control sequences(si-NC,miR-NC)were constructed,and transfected into Eca109 cells as si-circ_0056618 group,miR-1281 group,si-NC group and miR-NC group.Eca109 cells were co-transfected with si-circ_0056618 and miR-1281 inhibitor sequences(anti-miR-1281)or negative control sequences(anti-miR-NC)as the si-circ_0056618+anti-miR-1281 group and the si-circ_0056618+anti-miR-NC group.Cell viability,clone formation number,scratch healing rate and number of invasive cells were measured by Cell Counting Kit-8,clone formation assay,scratch assay and Transwell assay,respectively.Protein expression of E-cadherin(E-cad)and N-cadherin(N-cad)were detected by Western blot.Targeting relationship between circ_0056618 and miR-1281 was detected by dual luciferase report experiments.Results Compared with the adjacent tissues,the expression of circ_0056618 in esophageal cancer tissues was increased,while the expression of miR-1281 was decreased(P<0.05).Compared with the si-NC group,there were decreased cell viability,levels of circ_0056618 and N-cad,scratch healing rate,and numbers of clone formation and invasive cells but increased E-cad level in the si-circ_0056618 group(P<0.05).Compared with the miR-NC group,there were decreased cell viability,N-cad level,scratch healing rate and numbers of clone formation and invasive cells but increased levels of miR-1281 and E-cad in the miR-1281 group(P<0.05).Circ_0056618 targeted and negatively regulated miR-1281.Compared with the si-circ_0056618+anti-miR-NC group,there were increased cell viability,N-cad level,scratch healing rate and numbers of clone formation and invasive cells but decreased levels of miR-1281 and E-cad in the si-circ_0056618+anti-miR-1281 group(P<0.05).Conclusion Knockdown of circ_0056618 can inhibit the migration,proliferation,and invasion of esophageal cancer Eca109 cells by increasing the expression of miR-1281,providing a potential breakthrough for targeted therapy of esophageal cancer.