Clinical value of serum microRNA-369-3p and microRNA-296-5p expression in early diagnosis and prognostic evaluation of patients with differentiated thyroid carcinoma

DIAN Hui; HE Mingchang; LIU Shaofei

doi:10.3969/j.issn.1009-0460.2025.04.009

Abstract：Objective To explore the clinical value of serum microRNA(miR)-369-3p and miR-296-5p expression in early diagnosis and prognostic evaluation of differentiated thyroid carcinoma(DTC)patients.Methods A total of 98 DTC patients admitted to our hospital from January 2016 to January 2019 were gathered as the cancer group.According to the prognosis,the patients were grouped into disease-free survival group(n=86)and recurrence and metastasis group(n=12).Additionally,98 thyroid adenoma patients were included as the benign lesion group,and 86 healthy individuals who underwent physical examinations were as the control group.Real-time quantitative polymerase chain reaction was applied to measure the expression levels of serum miR-369-3p and miR-296-5p in each group,and their relationship with the clinical pathological characteristics of patients was analyzed.Receiver operating characteristic curve was applied to analyze the early diagnostic value of serum miR-369-3p and miR-296-5p in DTC.Kaplan-Meier method was applied to analyze the relationship between serum miR-369-3p and miR-296-5p expression levels and disease-free survival in DTC patients.Results Serum miR-369-3p and miR-296-5p expression levels were reduced in the control,benign lesion and cancer groups in that order[(1.00±0.26),(0.94±0.20),(0.64±0.15);(0.98±0.23),(0.77±0.15),(0.56±0.10)],and the differences between groups were statistically significance(F=83.238,147.577,P<0.05).Serum miR-369-3p and miR-296-5p expression levels were related to degree of differentiation,maximum tumor diameter,TNM stage,and lymph node metastasis(P<0.05).The area under the curve of serum miR-369-3p and miR-296-5p for individual and combined diagnosis of DTC was 0.864,0.878 and 0.946,and the combined diagnosis was superior to individual diagnosis(Zcombinationvs.miR-369-3p=3.308,Zcombinationvs.miR-296-5p=3.804,P<0.001).Serum miR-369-3p(0.48±0.04)and miR-296-5p(0.46±0.02)expression levels of patients in the recurrent and metastasis group were significantly lower than those in the disease-free survival group(0.67±0.17,0.58±0.11)(P<0.05).The 5-year disease-free survival rate of patients with high expression of serum miR-369-3p was greatly higher than that of patients with low expression(χ2=4.212,P=0.040),and the 5-year disease-free survival rate of patients with high expression of miR-296-5p was greatly higher than that of patients with low expression(χ2=4.403,P=0.036).Conclusion The serum levels of miR-369-3p and miR-296-5p in patients with DTC are decreased,both have high efficacy in early diagnosis and prognositic prediction of DTC,and have certain clinical value.