Relationship between HLA class I molecules and prognosis in acute myeloid leukemia with NPM1 mutation
LI Wenjun
LIU Aixi
CHEN Yaoyao
HAN QingRu
HAN Bing
Abstract：Objective To investigate the relationship between human leukocyte antigen(HLA)class I molecules and acute myeloid leukemia(AML)with nucleophosmin(NPM1)mutation and prognosis.Methods A total of 106 patients with NPM1-mutated AML and 90 patients with NPM1-negative AML treated in the 960th Hospital of the Joint Logistics Support Force of the People's Liberation Army from January 2018 to December 2021 were collected.Additionally,60 healthy volunteers without a blood relationship to AML patients and without hematological diseases during the same period were selected as a healthy control group.HLA class I genotyping was performed for all subjects using polymerase chain reaction-sequence specific primer method.The overall survival(OS)of the patients was recorded through follow-up.Predictive software tools were used to search for NPM1-derived immunopeptides.Results The frequencies of HLA class I alleles A02,B07,B40,and C07 in the NPM1-mutated AML group were significantly lower than those in the healthy control group,and the frequencies of B07 and C07 were significantly lower than those in the NPM1-negative group(P<0.05).Compared with AML patients who did not express specific HLA class I alleles(A02,B07,B40,C07),patients expressing at least one specific HLA class I allele had a lower mortality rate(P<0.05).Kaplan-Meier analysis showed that the median OS of HLA class I molecule-positive patients in NPM1-mutated AML was longer(P<0.001).Cox proportional hazards regression model analysis showed that FLT3-ITD negativity and HLA-B07 expression were independent factors affecting the OS of patients with NPM1-mutated AML(P<0.05).Only in patients negative for FLT3-ITD mutation,the survival prognosis of patients positive for HLA-B7 and HLA-C7 was significantly better than that of the negative group(P<0.05).Predictive software tools identified candidate NPM1-derived immunopeptides for B40 and B07.Conclusion The specific HLA class I alleles in NPM1-mutated AML patients are significantly reduced,and the presence of HLA-B07 or C07 antigens is associated with better survival in FLT3-ITD negative NPM1-mutated AML patients.
Keywords：Acute myeloid leukemiaNPM1 mutationHLA class I moleculesPrognosis
Publication Date：2025-03-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 244-250 )