Correlation analysis of FXYD3 expression with clinicopathological features and prognosis in patients with intrahepatic cholangiocarcinoma

YANG Aihua; SHEN Jianan; ZHOU Yan; WANG Chaoshan; DAI Tianyi; HE Xintao; CHEN Jun

doi:10.3969/j.issn.1009-0460.2025.03.003

Abstract：Objective To analyze the expression of FXYD3 in intrahepatic cholangiocarcinoma(ICC)patients and evaluate its value in histological subtypes and prognosis.Methods A retrospective analysis was conducted on 245 tumor samples obtained from ICC patients who underwent radical surgical resection at Nanjing Drum Tower Hospital between 2014 and 2024,with postoperative pathology confirming ICC.Clinical and pathological data,preoperative serum biochemical parameters,and follow-up information were collected.Based on cellular morphology and tissue architecture,ICC was classified into large-duct and small-duct subtypes.Immunohistochemical staining was used to assess and score FXYD3 expression in ICC tumor tissues.The Kaplan-Meier method was used to draw the overall survival(OS)curve;Cox proportional hazards regression model was used to analyze the factors affecting OS of patients.Results Among 245 patients,123 cases showed high FXYD3 expression,while 122 exhibited low expression.High FXYD3 expression was observed in 86.2%(81/94)of large-duct ICC cases,compared to 27.8%(42/151)of small-duct ICC cases,indicating a statistically significant difference in FXYD3 expression between the two histological subtypes(χ2=78.917,P<0.001).The Kaplan-Meier method showed that the 5-year survival rate of patients in the FXYD3 high expression group was 59.3%(73/123),which was lower than that in the FXYD3 low expression group(71.3%,87/122),and the difference was statistically significant(P=0.014).Multivariate Cox proportional hazards regression analysis identified FXYD3 expression as an independent prognostic factor for ICC patients(HR=1.722,95%CI:1.007-2.945,P=0.047).Conclusion FXYD3 is significantly expressed in large-duct type ICC,and high FXYD3 expression is an independent prognostic risk factor for ICC.