Role of SYT8 in promoting epithelial mesenchymal transition of colorectal cancer cells
LI Yan
ZHOU Tong
MIAO Yaru
LI Qing
HU Lina
WANG Jintao
BI Yanghui
Abstract：Objective To investigate the effect of SYT8 on the overall survival(OS)of patients with colorectal cancer,and its effect on colorectal cancer cell migration and epithelial mesenchymal transition(EMT).Methods The Kaplan-Meier method was used to analyze the effect of SYT8 expression on OS of colorectal cancer patients in the the Cancer Genome Atlas(TCGA)database,and the effect of SYT8 expression on survival of colorectal cancer patients with or without lymph node metastasis was compared by stratified analysis.Interference with SYT8 expression was achieved by transfection of knockdown lentivirus(siRNA-SYT8)in KM12,HT29 cells.The migration ability of colorectal cancer cells after transfection was detected by Transwell assay.The effect of SYT8 on the expression of markers related to EMT was detected by western blotting.The correlation of expression between SYT8 and EMT-related markers was analyzed by Pearson correlation.Results Analysis of the TCGA online database showed that high SYT8 expression was significantly associated with poor prognosis in colorectal cancer patients(P＜0.05).The OS was shorter in patients with high SYT8 expression in patients who developed lymph node metastases(P＜0.05).Reduced SYT8 expression inhibited the migratory ability of colorectal cancer cells KM 12 and HT29,and inhibited EMT of KM 12 cell line,which resulted in the increase of the expression of E-cadherin,and the decrease of the expression of N-cadherin,Vimentin and Snail1.In colorectal cancer data of TCGA database,SYT8 was negatively correlated with the gene CDH1 encoding E-cadherin(r=-0.206,P＜0.05)and positively correlated with Snail1 expression(r=0.118,P＜0.05).Conclusion High SYT8 expression is associated with poor prognosis in colorectal cancer patients.In colorectal cancer patients with lymph node metastasis,high SYT8 expression predicts poor prognosis.Reducing SYT8 expression inhibits the migration ability of colorectal cancer cells,and the mechanism of which may be by inhibiting the EMT of colorectal cancer cells.
Keywords：Colorectal cancerSYT8MetastasisEpithelial-mesenchymal transition
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 125-130 )