Effects of microRNA-487a targeting MAGI2 on cell migration and invasion in renal cell carcinoma

XU Huiyang; CHEN Bote; QIU Xiaofo

doi:10.3969/j.issn.1009-0460.2025.02.003

Abstract：Objective To explore the role of miR-487a in proliferation,migration and invasion in renal cell carcinoma.Methods The expression of miR-487a in human renal cancer cell lines(786-O,ACHN,Caki-1,and Caki-2)and immortalized normal human proximal tubule epithelial cell line HK-2 was detected by quantitative real-time PCR(qRT-PCR).786-O renal cancer cells were divided into three groups:NC group(transfected with miR-487a negative control),Control group(blank control),and miR-487a inhibitor group(transfected with miR-487a inhibitor).The effects of miR-487a on the proliferation of 786-O cell was evaluated by CCK-8.Wound-healing and Transwell assays were used to examine the migration and invasion abilities of renal cell carcinoma cells.The targeting relationship between miR-487a and MAGI2 was explored using luciferase reporter analysis.The expressions of MAGI2,MMP-9 and Smad4 were detected by qRT-PCR and western blotting analysis.Results Compared with HK-2 cell,miR-487a was up-regulated in renal cancer cell Caki-2,ACHN,Caki-1 and 786-O(P＜0.05).Compared with the control group cells,the proliferation ability of 786-O renal cancer cells transfected with miR-487a inhibitor after 48 and 72 hours were decreased,and the difference was statistically significant(P＜0.05).Compared with the NC group,the number of cell migration(25.847±3.126 vs.76.533±4.243)and the number of transmembrane cells(101.600±13.741 vs.274.200±54.348)in the miR-487a inhibitor group were significantly reduced(P＜0.05).MAGI2 may be a target gene of miR-487a.Compared with NC group,the expressions of MAGI2 and Smad4 were promoted,while MMP-9 was reduced in miR-487a inhibitor group(P＜0.05).Conclusion miR-487a can target MAGI2,inhibit Smad4 expression,and function as a tumor promoter in human renal cancer.