Expression and prognosis of AT rich interactive domain 5A in cervical cancer tissues
CHEN Xiaoyan
QIN Tao
WANG Haining
XU Shishen
BI Yiming
Abstract：Objective To investigate the expression of AT rich interactive domain 5 A(ARID5A)in cervical cancer tissues and its relationship with prognosis.Methods Bioinformatics and immunohistochemical EnVision two-step method were used to analyze the expression of ARID5A in cervical cancer tissues and its relationship with clinicopathological features,prognosis and immune cell infiltration level.Gene enrichment analysis of possible pathways involved in ARID5A was performed.Results Bioinformatics analysis showed that the ARID5A expression in cervical cancer tissues was lower than that in adjacent tissues(P＜0.001),and the overall survival of patients with low ARID5A expression was shorter than that of patients with high ARID5A expression(P=0.030).Immunohistochemistry confirmed that the low expression rate of ARID5A protein in cervical cancer tissues was 67.50％(54/80),higher than 23.75％(19/80)in adjacent tissues(P=0.007).Clinicopathological analysis showed that the ARID5A expression was correlated with FIGO stage,lymph node metastasis,vascular thrombus,differentiation degree,Ki67 and P16 expression in cervical cancer patients(P＜0.05).Survival analysis showed that the overall survival and disease-free survival in patients with the low ARID5A expression were significantly shorter than those in patients with high ARID5A expression group(P＜0.05).Cox regression analysis showed that the low expression and differentiation of ARID5A were independent risk factors for the prognosis of cervical cancer patients(P＜0.05).Immune cell infiltration analysis showed that the expression of ARID5A in cervical cancer was positively correlated with the infiltration levels of NK cells,macrophages and B cells.Gene enrichment analysis revealed that ARID5A may be involved in humoral immune response,cytokine-cytokine receptor interaction,nuclear receptor and peroxisome proliferator-activated receptor signaling pathways.Conclusion The low ARID5A expression in cervical cancer tissues is an independent risk factor affecting the prognosis of cervical cancer patients,and it is expected to be used as a molecular marker for the prognosis evaluation of cervical cancer.
Keywords：Cervical cancerAT rich interactive domain 5APrognosisClinical significance
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1225-1231 )