Clinical significance and biological function of SNW domain containing 1 in hepatocellular carcinoma
ZHANG Dan
SHAO Yang
LI Linlin
CHEN Kai
GUO Pan
ZHANG Hao
Abstract：Objective To identify the potential biomarker value of SNW domain containing 1(SNW1)in hepatocellular carcinoma through bioinformatics methods,and to explore the effect of SNW1 on the proliferation and invasion abilities of hepatocellular carcinoma cells through cell experiments.Methods Mendelian randomization combined with biostatistical methods were employed to identify characteristic genes with a causal relationship to hepatocellular carcinoma.Transcriptome differential expression and survival analysis were utilized to examine the expression of characteristic genes in hepatocellular carcinoma and the correlation of characteristic genes and prognosis,as well as to identify the potential of SNW 1 as a potential biomarker.Additionally,correlation analysis of SNW 1 with gene set enrichment and immune cell infiltration were performed.Furthermore,real-time fluorescence quantitative polymerase chain reaction was utilized to ascertain the SNW1 expression in human hepatocellular carcinoma cell lines HepG2 and Hep3B.SNW1 in the hepatocellular carcinoma cells was knocked down by cell transfection,followed by cell counting kit-8,cell scratch,and Transwell assays to detect the proliferation,migration,and invasion abilities of hepatocellular carcinoma cells.Results SNW1 had been identified as a potential risk factor for hepatocellular carcinoma,with significantly elevated expression levels observed in patients(P＜0.05).Additionally,SNW 1 expression levels were correlated with the prognosis of hepatocellular carcinoma patients(P＜0.05).The pathways significantly positively correlated with SNW1 mainly involved biological processes such as RNA splicing,viral carcinogenesis,and Epstein-Barr virus infection.SNW 1 was significantly positively correlated with quiescent dendritic cells and eosinophils,and negatively correlated with regulating T cells and activating NK cells.Cell experiments demonstrated that the knockdown of SNW1 inhibited the capacity of hepatocellular carcinoma cells to proliferate,migrate,and invade.Conclusion SNW1 has the potential to serve as a novel biomarker and therapeutic target for hepatocellular carcinoma.
Keywords：Hepatocellular carcinomaSNW domain containing 1Mendelian randomizationClinical significance
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 1217-1224 )