Levels and clinical significances of protein kinase N1 and protein kinase N2 in breast cancer tissues
BAO Yanhong
GAO Fan
WANG Lei
LIANG Xueyao
LIU Xiaohua
Abstract：Objective To investigate the levels and clinical significance of protein kinase N1(PKN1)and protein kinase N2(PKN2)in breast cancer tissues.Methods Real-time quantitative PCR(qPCR)was used to detect the levels of PKN1 and PKN2 in 94 pairs of breast cancer tissues and adjacent tissues.Clinical significance of both levels in breast cancer were investigated using clinical and pathological characteristics,and the impact of both levels on the prognosis of breast cancer was analyzed online with the help of Kaplan-Meier Plotter database.Results The levels of PKN1 and PKN2 in breast cancer tissues were 4.804±1.750 and 4.376±1.611,higher than 1.743±0.912 and 1.670±0.850 in adjacent tissues,and the difference was statistically significant(t=15.045,14.402,P＜0.001).Pearson correlation analysis showed that the levels of PKN1 and PKN2 in breast cancer tissues were positively correlated(r=0.605,P＜0.001).Stratified analysis revealed a positive correlation between tissue PKN1 level and tumor size(r=0.209,P=0.038),lymph node metastasis(r=0.200,P=0.048),TNM staging(r=0.304,P=0.002),histological grading(r=0.313,P=0.001),and Nottingham prognostic index(r=0.297,P=0.011).The level of PKN2 was positively correlated with tumor size(r=0.297,P=0.003),lymph node metastasis(r=0.309,P=0.002),and TNM staging(r=0.430,P＜0.001).Kaplan-Meier Plotter online analysis showed that PKN1 was related to the recurrence free survival(HR=0.85,95％CI:0.77-0.94)and overall survival(HR=0.79,95％CI:0.65-0.95)of breast cancer,while PKN2 was only related to the recurrence free survival(HR=0.77,95％CI:0.70-0.85)of breast cancer.Conclusion PKN1 and PKN2 are highly expressed in breast cancer,participate in the malignant progression of breast cancer and are related to recurrence.Both are expected to become potential targets for prevention and treatment of breast cancer.
Keywords：Breast cancerProtein kinase N1Protein kinase N2Clinical significancePrognosis
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1200-1204 )