EffectsI of ITGB2 antisense RNA 1 on the sensitivity of non-small cell lung cancer cells to cisplatin
CHEN Huiyong
WANG Kailei
WAN Renping
LI Youtao
WANG Linhui
Abstract：Objective To investigate the role of ITGB2 antisense RNA 1(ITGB2-AS1)on cisplatin drug sensitivity in non-small cell lung cancer cells.Methods Paracancerous and cancerous tissues from non-small cell lung cancer patients and cisplatin-resistant patients were collected,and the expression level of ITGB2-AS1 in the tissues was detected by fluorescence in situ hybridization.Cisplatin-resistant cell lines A549/DDP and H1975/DDP were cultured,and ITGB2-AS1 expression was detected by real-time fluorescence quantitative PCR(qPCR)in A549/DDP and H1975/DDP cells.Small hairpin RNA sequence targeting ITGB2-AS1(sh-ITGB2-AS1)and a negative control sequence(sh-NC)were constructed and transfected into A549/DDP and H1975/DDP cells.Cells were divided into Control group,Cisplatin group,Cisplatin+sh-NC group,and Cisplatin+sh-ITGB2-AS1 group.Moreover,qPCR was performed to detect interference efficiency of sh-ITGB2-AS1.Cell viability and apoptosis levels were detected by CCK-8 and TUNEL assays.Cell abilities of migration and invasion were detected by Transwell assay,and multidrug resistance protein 1(MRP1)and P-glycoprotein(P-gp)expression levels were detected in cells by Western blot.Results ITGB2-AS1 expression levels were elevated in cancer tissues compared with paracancerous tissues,and were highest in cancer tissues of chemoresistant patients.Compared with A549 and H1975 cells,ITGB2-AS1 expression levels were elevated in A549/DDP and H1975/DDP cells.Transfection of sh-ITGB2-AS1 inhibited ITGB2-AS1 expression levels in A549/DDP and H1975/DDP cells.There were no significant changes in cell viability,apoptosis level and abilities of migration and invasion in the Cisplatin and Cisplatin+sh-NC groups compared to the Control group.The decreased cell viability,elevated apoptosis level and weakened abilities of migration and invasion were found in Cisplatin+sh-ITGB2-AS1 group as compared with Cisplatin+sh-NC group.Expression levels of MRP1 and P-gp proteins were decreased in the cells of Cisplatin+sh-ITGB2-AS1 group compared with Control,Cisplatin and Cisplatin+sh-NC groups.Conclusion ITGB2-AS1 expression is upregulated in lung cancer,and inhibition of ITGB2-AS1 enhances the sensitivity of cisplatin-resistant cells to cisplatin.
Keywords：Non-small cell lung cancerITGB2 antisense RNA 1CisplatinDrug resistance
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1152-1158 )