Expression and clinical significance of DJ-1 protein and monoacylglycerase in prostate cancer

SUN Chaoqun; LIN Lei; YANG Shuli; HAO Yang; ZHAO Xiaofeng

doi:10.3969/j.issn.1009-0460.2024.08.003

Abstract：Objective To observe the expression of human protein DJ-1(DJ-1)and monoacylglycerase(MGLL)in prostate cancer tissues,and analyze their relationship with clinicopathological features and prognosis of prostate cancer.Methods A total of 112 patients diagnosed with prostate cancer by clinical puncture from August 2019 to August 2021 were enrolled as the study objects.Western blotting method was used to detect the expression level of DJ-1,MGLL in fresh cancer tissues and adjacent tissues.The relationship between clinicopathological features of prostate cancer and DJ-1,MGLL was analyzed.Receiver operating characteristic(ROC)curve was used to evaluate the cut-off of DJ-1 and MGLL at relapse or death.Postoperative patients were followed-up to August 2023.The correlation between DJ-1 and MGLL expression and the 2-year recurrence-free survival rate of prostate cancer patients was further analyzed.Results The relative expression levels of DJ-1 and MGLL in the adjacent tissues of prostate cancer were 0.23±0.03 and 0.84±0.09,and those in cancer tissues were 0.70±0.09 and 0.22±0.06,with statistically significant difference(P＜0.05).The expression levels of DJ-1 and MGLL in prostate cancer tissues were correlated with TNM stage and lymph node metastasis(P＜0.05),but not with age,tumor diameter and Gleason score(P＞0.05).ROC curve showed that the cut-off of DJ-1 and MGLL was 0.48 and 0.22 respectively at relapse or death.All 112 prostate cancer patients were followed-up after surgery,and the 2-year relapse-free survival rate of prostate cancer patients with high DJ-1 expression was 65.6％,lower than 93.8％of patients with low DJ-1 expression(P=0.033).The 2-year relapse-free survival rate of prostate cancer patients with low MGLL expression was 65.2％,lower than 90.0％of prostate cancer patients with high MGLL expression(P=0.041).Conclusion The expression levels of DJ-1 and MGLL are correlated with TNM stage,lymph node metastasis,and relapse-free survival in prostate cancer patients.