The effect of atractylodes macrocephalaon polysaccharide on stem cell-like properties of hepatoma cells,epithelial-mesenchymal transition and phosphorylation of Akt/NF-κB pathway
LI Chen
DU Liming
SUN Qiu
LI Guangjian
HU Yali
Abstract：Objective To explore the effects of atractylodes macrocephalaon polysaccharide(AMPs)on cell-like properties of hepatoma cells,epithelial-mesenchymal transition(EMT)and phosphorylation of Akt/NF-κB.Methods The hepatoma cells HepG2 were cultured and randomly divided into blank control group,low-concentration,medium-concentration and high-concentration AMPs groups(50,100,200 μg/ml).The cells pelletization was observed under microscope.The expression of growth markers,stem cell-like markers and Akt/NF-κB pathway in HepG2 was detected by Western blotting.The expression of EMT markers in hepatoma cells was detected by real-time fluorescent quantitative PCR and immunofluorescence.Results Compared with the blank control group,treatment with AMPs at concentrations of 100 and 200 µg/ml resulted in down-regulation of Ki-67 and PCNA protein expression and up-regulation of p21 protein expression in HepG2 cells(P＜0.05);The number of cell spheroids and the diameter of spheroids significantly decreased(P＜0.05);The expression of SOX2,Oct4,and Nanog proteins was down-regulated(P＜0.05);The expression level of E-cad mRNA increased,while the expression level of N-cad mRNA and the number of Vimentin positive stained cells decreased(P＜0.05);The phosphorylation levels of Akt and p-P65 NF-κB decreased(P＜0.05).There was no statistically significant difference in the above indicators between the 50 µg/ml AMP group and the blank control group(P＞0.05).Conclusion AMPs can inhibit the activity of HepG2,stem cell-like properties and EMT ability.The inhibition of Akt/NF-κB phosphorylation may be its potential action mechanism.
Keywords：HepatomaAtractylodes macrocephalaon polysaccharideStem cell-like propertyEpithelial-mesenchymal transitionAkt/NF-κB signaling pathway
Publication Date：2024-08-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 735-739 )