Mechansism of miR-139-5p on inhibiting proliferation,migration and invasion of gastric cancer cells by targeting COL11A1
MA Tingting
ZHOU Liqing
SU Xinyu
XIA Jianhong
YAN Yan
Abstract：Objective To explore the function and potential molecular regulatory mechanisms of miR-139-5p in gastric cancer(GC)progression.Methods TCGA-STAD expression data was downloaded from TCGA database for differential analysis to determine the target miRNA(miR-139-5p).The downstream regulatory target genes of the miRNA were predicted using the BioSignal database,and the target mRNA(COL11A1)was identified by taking the intersection with the differentially expressed mRNA.GC cells were divided into the following subgroups:NC mimics,miR-139-5p mimics,NC mimics+oe-NC,miR-139-5p mimics+oe-NC,and miR-139-5p mimics+oe-COL11A1.qPCR was performed to detect the expression of mRNA in each group.CCK-8 assay and clone formation assay were performed to detect the proliferative ability.Transwell assay was performed to detect the invasive and migratory ability,and Western blotting was performed to detect the the expression of MMP-2 and MMP-9.Dual luciferase reporter gene assay was used to verify the binding relationship between miR-139-5p and COL11A1.Results The database analysis and qPCR detection results indicated that miR-139-5p was significantly under-expressed in GC tissues and cells,and overexpression of miR-139-5p could inhibit the proliferation,migration and invasion ability of GC cells.Dual luciferase reporter gene assay confirmed that the downstream regulatory target gene of miR-139-5p was COL11A1,and COL11A1 was significantly highly expressed in GC cells.Rescue experiments showed that overexpression of miR-139-5p could inhibit the proliferation,migration and invasion ability of GC cells,while overexpression of COL11A1 could reverse the inhibitory effect of over-expression of miR-139-5p on GC cells.Conclusion miR-139-5p is able to regulate the proliferation,invasion and migration of GC cells by targeting COL11A1 thereby.
Keywords：Gastric cancerMiR-139-5pCOL11A1ProliferationMigrationInvasion
Publication Date：2024-08-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 729-734 )