Correlation analysis of COL11A1 expression with ER,PR,HER-2 and Ki-67 in breast cancer tissues
JI Pan
YANG Yunbo
BAI Rumeng
HE Xiao
LYU Junwen
MIAO Chen
Abstract：Objective To investigate the relationships of collagen type XI alpha 1 chain(COL11A1)expression with estrogen receptor(ER),progesterone receptor(PR),human epidermal growth factor receptor-2(HER-2),and Ki-67 in breast cancer tissues.Methods Eighteen cases of breast fibroadenoma and 120 cases of breast cancer tissues(36 cases of carcinoma in situ and 84 cases of invasive carcinoma)were collected.The expression of COL11A1 protein was detected by immunohistochemical EnVision two-step method.Immunohistochemical detection of ER/PR/HER-2/Ki-67 was performed on breast cancer tissues,and FISH detection was performed on HER-2(2+).Relationships of COL11A1 protein with clinicopathologic features of breast cancer and various immune markers were analyzed.Results Immunohistochemical result showed that COL11A1 was mainly located in breast cancer cells.COL11A1 expression was weakly positive in fibriadenoma samples.COL11A1 expression in carcinoma in situ was significantly higher than that in invasive breast cancer(P<0.05).The COL11A1 expression was uncorrelated with the age,location,tumor size and lymph node metastasis of breast cancer patients(P>0.05).The COL11A1 expression in the medium-high differentiation tissues was significantly higher than that in the low-differentiation tissues(P<0.05).Further analysis of the correlation between COL11A1 and other breast cancer immune markers showed that the proportion of COL11A1-low expression was higher in Ki-67 high tissues with statistical significance(P<0.05),while no significant correlations were observed between the other three markers and COL11A1 expression(P>0.05).Conclusion The COL11A1 expression in invasive breast cancer is lower than that in carcinoma in situ,and the expression level in low-differentiated breast cancer is lower than that in medium-differentiated breast cancer.COL11A1 is correlated with the expression of Ki-67,suggesting that COL11A1 expression changes dynamically during the occurrence and progression of breast cancer.This study provides a potential marker for the diagnosis and treatment of breast cancer.
Keywords：Breast cancerCollagen type XI alpha 1 chainEstrogen receptorProgesterone receptorHuman epidermal growth factor receptor-2Ki-67
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1004-1007 )