Effects of GK-IT1 on proliferation,migration and invasion of gastric cancer cells via JAK signaling pathway
WANG Qiong
LI Hui
WU Yongbin
Abstract：Objective To examine the effects of long non-coding RNA GK intronic transcript 1(GK-IT1)on the biological activities of gastric cancer cells via the Janus kinase(JAK)signaling pathway.Methods UALCAN was applied to analyze the expression of GK-IT1 in gastric cancer tissues.Expressions of GK-IT1 in human gastric cancer cell lines were evaluated by quantitative real-time polymerase chain reaction(qPCR)assay.BSG-823 cells were treated with small interference RNA(siRNA)against GK-IT1 si-GK-IT1,or si-GK-IT1 and Coumermycin A1(an activator of the JAK2 signaling pathway)both in combination,and allocated into Negative control group,si-GK-IT1 group and si-GK-IT1+JAK2 group.Cell proliferation,migration and invasion were evaluated by CCK-8,scratch and Transwell assays.Expressions of B-cell lymphoma-2(Bcl-2),cleaved-caspase-3(CCP3),matrix metallopeptidase-9(MMP-9)and phosphorylated Janus-activated kinase 2(p-JAK2)were tested by qPCR or Western blot assays.Results GK-IT1 was upregulated in gastric cancer tissues and related with tumor stage and grade(P<0.05).Gastric cancer cells showed higher expression of GK-IT1 compared to the findings in gastric mucosal epithelial RGM-1 cell(P<0.05).Moreover,si-GK-IT1 group exhibited blunted abilities of proliferation,migration and invasion compared to Negative control group(P<0.05),whereas si-GK-IT1+JAK2 group reversed these results(P<0.05).Compared with the Negative control group,the expression of Bcl-2,MMP-9 and p-JAK2 in the si-GK-IT1 group decreased,while expression of CCP3 in the si-GK-IT1 group increased(P<0.05).Compared with the si-GK-IT1 group,in addition to the decreased expression of CCP3,the si-GK-IT1+JAK2 group showed an increase in the expression of the rest factors(P<0.05).Conclusion GK-IT1 regulates the proliferation,migration,and invasion of gastric cancer cells through inhibition of the JAK signaling pathway,and is expected to become a potential target for gastric cancer treatment.
Keywords：Gastric cancerGK intron transcript 1ProliferationInvasion and migrationJAK signaling pathway
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 974-979 )