Serum level and clinical significance of BARD1 in patients with gastric cancer

YANG Jing; JING Xiaosong; WANG Yingwei

doi:10.3969/j.issn.1009-0460.2019.11.013

Abstract：Objective To investigate the level of BRCA1-associated ring domain protein 1 (BARD1) in serum of patients with gastric cancer and its clinical significance. Methods The BARD1 levels in gastric cancer tissues and normal tissues were analyzed online by GEPIA database, and serum BARD1 levels of 177 gastric cancer patients and 173 healthy physical examinees in our hospital from Jan-uary 2014 to December 2017 were detected by real-time quantitative PCR (QPCR). Relationship between serum BARD1 level and clini-copathological characteristics of gastric cancer was analyzed. According to the follow-up data, the relationship between the serum BARD1 level and prognosis of gastric cancer was analyzed. Results On-line analysis of GEPIA database showed that the median level of BARD1 in 408 cases of gastric cancer was 3. 87, higher than 1. 36 in 36 cases of normal tissues (P＜0. 05). The results of QPCR showed that the serum BARD1 level of 177 patients with gastric cancer was 4. 034±1. 941, higher than 1. 427±0. 799 of the healthy physical examinees (t＝16. 367, P＜0. 001). The serum level of BARD1 in gastric cancer was not related to gender, age, tumor location and invasion depth (P＞0. 05), but related to tumor diameter, differentiation degree, Bormann classification, lymph node metastasis, distant metastasis and TNM stage (P＜0. 05). The median overall survival was 39. 0 months in the low-level group, longer than 36. 0 months in high-level group (P＜0. 05). Multivariate analysis showed that serum BARD1 level was an independent factor influencing the prognosis of gastric cancer (OR＝3. 145, 95% CI: 2. 897-4. 268, P＝0. 017). Conclusion The expression of BARD1 is up-regulated in the tissues and serum of patients with gastric cancer. The serum level of BRDA1 is related to the malignant degree and prognosis of gastric cancer, suggesting that BARD1 may be related to the occurrence and development of gastric cancer.