Effect of microRNA-422b on invasion, migration and JAK1／STAT3 signaling pathway of glioma cells
GE Feng
JIANG Yunzhao
WANG Yong
CHEN Dongdong
Abstract：Objective To investigate the influence of microRNA-422b (miR-422b) on migration, invasion and Janus kinase 1(JAK1)／transduction and the activators of the transcription 3 ( STAT3) signaling pathway of human glioma cells. Methods Real-time quantitative PCR (QPCR) was used to detect expressions of miR-422b in human astrocytes HA1800 and glioma cells U-87. Lipi-dosome was used to mediate miR-422b inhibitor ( Inhibitor group) and negative control ( NC group) transfecting u-87 cells and un-transfected cells were used as the Control group. QPCR, scratch test and Transwell test were used to detect miR-422b level, scratch healing rate and number of membrane penetrating cells of U-87 cells. Western blotting was used to detect levels of phosphorylated JAK1 ( p-JAK1) and phosphorylated STAT3 (p-STAT3). Results Compared with HA1800 cells (1. 029±0. 047), miR-422b level in U-87 cells increased to 3. 698±0. 274 (P＜0. 05). The miR-422b level, scratch healing rate and the number of membrane penetrating cells in Inhibitor group were 0. 264±0. 065, (25. 327±5. 320)% and 75. 826±10. 639, lower than 1. 169±0. 354, (59. 856± 6. 831)% and 183. 742±15. 736 in Control group and 1. 175±0. 262, (62. 947±7. 850)% and 175. 981 ±12. 560 in NC group (P＜0. 05). Compared with the Control group and NC group, levels of p-JAK1 and p-STAT3 in Inhibitor group decreased (P＜0. 05). No significant difference was observed between Control group and NC group (P＞0. 05). Conclusion The high expression of miR-422b can promote the migra-tion and invasion of glioma cells, which can be a candidate target for gene therapy of glioma.
Keywords：GliomaMicroRNA-422bMigrationInvasion
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1004-1007 )