Expression and clinical significance of PD?1／PD?L1 and CD8+Tils in cervical squamous cell carcinoma

SONG Chen; LIANG Gang; SHI Jiangbo; ZHENG Huixia

doi:10.3969/j.issn.1009-0460.2019.09.009

Abstract：Objective To investigate the relationship between the expression of PD?1／PD?L1 and PD?L1 combined with the density of CD8+tumor infiltrating lymphocytes ( Tils) and the clinicopathological parameters and prognosis in patients with cervical squamous cell carcinoma (SCC).Methods The expression of PD?1／PD?L1 and the density of CD8+Tils in 131 cases of cervical squa?mous cell carcinoma were detected by immunohistochemical staining from 2008 to 2017 in the Department of Pathology, First Hospital of Shanxi Medical University. The relationship between the expression of PD?1 and PD?L1 and clinicopathological features in cervical squamous cell carcinoma and the effect of the combination of PD?L1 and CD8+Tils density on the prognosis of patients with cervical squamous cell carcinoma was analyzed. Kaplan?miere method was used to draw survival curve and parallel Log?rank test. Cox propor?tional regression model was used to analyze the prognostic factors. Results The positive rate of PD?L1 was 64. 89%. The positive rate of PD?L1 was significantly correlated with the depth of interstitial invasion, FIGO stage, differentiation degree, lymphatic space inva?sion, lymph node metastasis and parauterine invasion (P＜0. 05). The positive rate of PD?1 was 61. 07% and the expression of PD?L1 was significantly correlated with the density of CD8+Tils. The PD?L1／CD8+Tils density group was associated with tumor size, FIGO stage and lymphatic space invasion (P＜0. 05). Patients with negative PD?L1 expression had a better prognosis than those with PD?L1 positive expression (P＜0. 05). The prognosis of patients with high density expression of CD8+Tils was better than that of patients with low density expression of CD8+Tils ( P＜0. 05).The prognosis of PD?L1－／CD8+Tils high density group was the best,the prognosis of PD?L1+／CD8+Tils low density group was the worst ( P＜0. 05). There was no significant difference in PD?1 expression and prognosis ( P＞0. 05). Multivariate analysis showed that the degree of differentiation, lymphatic space invasion, lymph node metastasis and FIGO staging were independent factors affecting OS in patients with squamous cell carcinoma of the cervix. Conclusion The expression of PD?1／PD?L1 in cervical squamous cell carcinoma is related to the density of CD8+Tils cells. PD?L1 combined with CD8+Tils density can evaluate the prognosis of cervical squamous cell carcinoma. To provide a new therapeutic target for clinical.