The effect of miR?29c?5p on the proliferation and migration of clear cell renal carcinoma cells by negatively regulating CPEB4
XU Zhihua
WANG Dong
Wang Jin
Abstract：Objective To investigate the negative regulation of miR?29c?5p on cytoplasmic polyadenylation primitive binding protein 4 (CPEB4) and its effect on the proliferation and migration of renal clear cell carcinoma Caki?1 cells. Methods miR?NC (miR?NC group), miR?29c?5p mimics ( miR?29c?5p mimics group), miR?29c?5p inhibitor ( miR?29c?5p inhibitor group) and miR?29c?5p inhibitor+si?CPEB4 (si?CPEB4+miR?29c?5p inhibitor group) were transfected into Caki?1 cells by LipofectamineTM 2000. MTT assay and Transwell assay were used to detect the effects of cell proliferation and migration activity in each group. Real?time fluores?cence quantitative PCR (QPCR) and Western blotting were used to detect the expression levels of miR?29c?5p, CPEB4 mRNA and CPEB4 protein in each group.Results The proliferation and migration activity of the cells in the miR?29c?5p mimics group was signif?icantly lower than that in the miR?NC group (P＜0. 05); while the proliferation and migration activity of cells in the miR?29c?5p inhibi?tor group was significantly higher than that in the miR?NC group (P＜0. 05).The proliferation and migration activity of cells in the miR?29c?5p inhibitor group was significantly higher than that in the miR?NC group (P＜0. 05). The expression levels of CPEB4 in the miR?29c?5p mimics group were significantly lower than those in the miR?NC group and the miR?29c?5p inhibitor group (P＜0. 05). The ex?pression levels of CPEB4 in the miR?29c?5p inhibitor group were significantly higher than those in the miR?NC group and the miR?29c?5p mimics group ( P＜0. 05). After silencing the expression of CPEB4 gene, the expression of miR?29c?5p of miR?29c?5p inhibitor group increased significantly (P＜0. 05).Compared with the miR?29c?5p inhibitor group, the proliferation and migration activity of cells in the si?CPEB4+miR?29c?5p inhibitor group decreased significantly ( P＜0. 05). Conclusion There are significant evidences that miR?29c?5p could inhibit the proliferation of clear cell renal cell carcinoma by down?regulation CPEB4 expression.
Keywords：Clear cell renal cell carcinoma(ccRCC)miR?29c?5pCytoplasmic poly?adenylation element binding pro?tein 4(CPEB4)ProliferationMigration
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 785-790 )