Changes of ER, PR, HER?2 and WT1 protein expression during the development of breast cancer

LIU Jing; ZHONG Ling; CHEN Qingqiu; ZHANG Kongyong; ZHANG Yi; JIANG Jun; QI Xiaowei

doi:10.3969/j.issn.1009-0460.2019.08.010

Abstract：Objective To explore the effect and mechanism of estrogen receptor (ER), progesterone (PR), human epider-mal growth factor receptor-2 (HER-2) and Wilms?? tumor gene 1(WT1) in carcinogenesis and development in breast cancer, so as to provide new ways of molecular targeted therapy. Methods Totally 95 patients from Jan 2011 to Dec 2011 (22 ductal epithelial hyper-plasia, 23 ductal epithelial atypical hyperplasia, 23 intraductal carcinoma and 27 intraductal infiltrating carcinoma) who were diag-nosed definitely as breast diseases with histological examination were included. Expression levels of ER, PR, HER-2 and WT1 were detected using immunohisitochemistry method and analyzed by relative analysis. Results ER and PR expression had no significant difference between both groups.The positive expression rates of HER-2 in ductal epithelial hyperplasia, ductal epithelial atypical hyper-plasia, intraductal carcinoma and intraductal infiltrating carcinoma were 0%(0／22), 4. 3%(1／23), 34. 8%(8／23) and 51. 9%(14／27), with the increasing trend(P＜0. 001). The positive expression rate of WT-1 in these four groups were 31. 8%(7／22), 60. 9%(14／23), 13. 0%(3／23) and 22. 2%(6／27) with the highest expression in atypical hyperplasia(P＝0. 002). In the intraductal car-cinoma patients, the expression of ER and PR(+／－) were associated with the positive ratio of WT-1 ( P＜0. 05). There were higher positive ratios of WT-1 in patients with positive ER or PR. Conclusion The gradually increase of ER and PR positive ratios indicated carcinogenesis and development of breast cancer. HER-2 participated in the initial process of breast cancer. WT-1 acted both as an on-cogene and the factor of invasion and migration, which promoted breast cancer evolution.