Expression and clinical significance of RNF216 in hepatocellular carcinoma tissues
GUO Jiang
LI Honglu
LI Changqing.
Abstract：Objective To investigate the expression of ring finger protein 216 (RNF216) in hepatocellular carcinoma (HCC) tis?sues and its relationship with clinical significance.Methods Data of gene expression and clinical characteristics of HCC patients were down?loaded from the Cancer Genome Atlas (TCGA).The relationship between RNF216 expression and clinicopathological characteristics and prog?nosis of HCC was analyzed.The relationship between RNF216 expression and prognosis of HCC patients was further analyzed using Kaplan?Meier Plottor online database.Using gene ontology (GO) analysis and functional enrichment analysis of Kyoto Encyclopedia of Genes and Ge?nomes (KEGG), we screened the biological functions or pathways that RNF216 may participate in and mainly influence. Results The ex?pression of RNF216 was related to clinical stage, T stage, M stage and overall survival (P＜0. 05), but not with age, N stage, surgical meth?od and radiotherapy (P＞0. 05).The online prognostic analysis of Kaplan?Meier Plottor showed that the high?expression of RNF216 was asso?ciated with shorter relapse?free survival (HR＝1. 52, 95%CI: 1. 09?2. 11), progression?free survival (HR＝1. 51, 95%CI: 1. 12?2. 02), dis?ease?specific survival (HR＝1. 95, 95% CI: 1. 21?3. 16) and overall survival (HR＝1. 79, 95%CI: 1. 23?2. 61) with statistical significance (P＜0. 05).Thirteen highly associated genes of RNF216 were screened with Spearman correlation coefficient＞0. 5 as a significant criterion:FOXK1, ANKRD52, CCDC93, TNRC18, C7orf70, RNF216L, HEATR2, USP42, POM121C, KCTD7, FBXL18, MED12 and ZMYM3.Fur?ther GO analysis revealed that RNF216 and its co?expressed genes were mainly components of cell membrane and nucleus, mostly binding to proteins, ions and nucleic acids, having catalytic activity and transferase activity, and involved in various biological regulation and metabolic processes.RNF216 high?expression samples were enriched to Hedgehog, Notch, ErbB and Wnt signaling pathway gene sets. Conclusion The expression of RNF216 in HCC tissues is related to many clinicopathological features, and RNF216 expression is a bad prognostic factor in HCC, which can be used as a potential molecular marker for evaluating the prognosis of HCC.
Keywords：Hepatocellular carcinoma(HCC)Ring finger protein 216(RNF216)Clinical significancePrognosis
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 605-609 )