Effects of isocryptotanshinone on proliferation, cycle and apoptosis of gastric cancer cells
HE Jiajie
LI Min
TANG Guofu
LI Houze
QI Xiaolong
Abstract：Objective Effects of isocryptotanshinone (ICTS) on proliferation, cycle and apoptosis of gastric cancer cell lines BGC-823 and SGC-7901 and its possible mechanism. Methods Gastric cancer cell lines BGC-823 and SGC-7901 were cultured in vitro. After treatment of 0, 5, 10, 20 μmol/L ICTS for 24, 48 and 72 hours, CCK-8 method was used to detect the inhibition rate of cell proliferation. After determining the optimal concentration and time of ICTS inhibiting BGC-823 and SGC-7901 cells, cell cycle and apoptotic rate were detected by flow cytometry. Western blotting was used to detect the expression of Cyclin D1, Bcl-2, p53 and p21 proteins in gastric cancer cells treated with ICTS. Results The inhibition rates of BGC-823 cells treated with 0, 5, 10, 20 μmol/L ICTS for 24 hours were (0. 789± 0. 048) ％, (16. 74± 1. 55) ％, (33. 58± 2. 26) ％ and (54. 62± 2. 61) ％. The difference was statistically significant (P＜ 0. 05). The inhibition rates of SGC-7901 cells treated with 0, 5, 10, 20 μmol/L ICTS for 24 hours were (-0. 184 ±0. 023) ％, (12. 76±1. 73) ％, (32. 95± 2. 47) ％ and (53. 80± 2. 65) ％. The difference was statistically significant (P＜ 0. 05). The inhibition rates of BGC-823 cells treated with 20 μmol/L ICTS at 24, 48 and 72 hours were (54. 62± 2. 61) ％, (55. 08± 2. 35) ％, (59. 72±2. 53) ％. There was no significant difference (P＞0. 05). The inhibition rates of SGC-7901 cells treated with 20 μmol/L ICTS at 24, 48 and 72 hours were (53. 80± 2. 65) ％, (55. 76± 2. 47) ％ and (61. 83± 2. 82) ％,. There was no significant difference (P＞0. 05). BGC-823 and SGC-7901 cells were treated with 20 μmol/L ICTS for 24 hours. The percentage of G0/G1 phase cells was (78. 34±7. 13) ％ and (79. 57±7. 34) ％, which were higher than those of the control group (P＜0. 05). The apoptotic rates of BGC-823 and SGC-7901 in ICTS treatment group were (24. 78±3. 42) ％ and (28. 76±4. 21) ％, which were higher than those of control group (P＜0. 05). The expression of Cyclin D1 and Bcl-2 protein in ICTS treatment group was significantly lower than that in the control group (P＜0. 05), and the expression of p53 and p21 protein in ICTS treatment group was significantly higher than that in the control group (P＜0. 05). Conclusion ICTS plays an anti-gastric cancer role by increasing the expression of p53 and p21, reducing the expression of Cyclin D1 and Bcl-2, thereby inhibiting cell proliferation, increasing G0/G1 blockade and apoptosis.
Keywords：Gastric cancerIsocryptotanshinone (ICTS)ProliferationApoptosis
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 129-132 )