Long chain non-coding RNA MEG3 targeting Rac1 inhibits proliferation and migration of cervical cancer cells

WANG Bing; XU Zhen; ZHAO Hu; ZHANG Hailing; WANG Wuliang; ZHOU Xiaoshui

doi:10.3969/j.issn.1009-0460.2019.02.005

Abstract：Objective To detect the expression of long chain non-coding RNA MEG3 in cervical cancer cells, and to detect the effect of MEG3 on the proliferation and migration of He La cells and its possible molecular mechanism. Methods The expression of MEG3 in normal cervical epithelial cells Hcer Epic and cervical cancer cell lines C-33 A, C4-1, Caski, Si Ha and He La was detected by fluorescence quantitative PCR (QPCR). MEG3 overexpression plasmid (MEG3 group), negative control plasmid (NC group) and MEG3+Rac1 overexpression plasmid (MEG3+Rac1 group) were transfected into He La cells respectively. MTT assay was used to detect cell proliferation and Transwell assay was used to detect cell migration. The expression of PCNA, E-cadenin, N-cadenin, Vimentin and Rac1 was detected by Western blotting assay. Results The expression levels of MEG3 in C-33 A, C4-1, Caski, Si Ha and He La cells were 0. 37±0. 044, 0. 65±0. 075, 0. 41±0. 071, 0. 71±0. 053 and 0. 42±0. 081, which were lower than those in Hcer Epic cells (P＜0. 05). MTT assay showed that the proliferation activity of He La cells in MEG3 group was significantly lower than that in NC group. The number of migrating cells in MEG3 group was 385±14, which was significantly lower than that in NC group (594±16), and the difference was statistically significant (P＜0. 05). Compared with NC group, the expressions of PCNA, N-cadenin and Vimentin were downregulated and E-cadenin was up-regulated in MEG3 group. Compared with MEG3 group, MEG3+Rac1 group significantly increased the expression of Rac1 protein, the proliferation activity and the number of migrating cells. Conclusion LncRNA MEG3 inhibits the proliferation and migration of cervical cancer cells by targeting Rac1 signaling pathway.