Effects of lncRNA TUG1 on proliferation, migration and energy metabolism of OVCAR3 cells

XU Sujuan; FU Ziyi; LIU Siyu; XU Pengfei

doi:10.3969/j.issn.1009-0460.2019.01.005

Abstract：Objective To investigate the expression of lncRNA TUG1 in ovarian cancer cells and its effects on proliferation, migration and energy metabolism of ovarian cancer OVCAR3 cells. Methods The expression of TUG1 in ovarian epithelial cancer cells (A2780, SKOV3, OVCAR3) and normal ovarian epithelial cells (IOSE80) was detected by quantitative fluorescence polymerase chain reaction (QPCR). TUG1 interference fragment was synthesized and transfected into OVCAR3 cells (interference group), and the negative control group (NC group) was set up. QPCR was used to verify the interference efficiency of TUG1. CCK-8 assay was used to detect the cell proliferation, Transwell and scratch assay were used to detect the cell migration, and seahorse energy analyzer was used to detect the changes of cell glycolysis pressure and mitochondrial pressure. Results QPCR results showed that the expression of TUG1 in ovarian cancer SKOV3, OVCAR3 cells was significantly higher than that in normal ovarian cells IOSE80 (P ＜ 0. 05).CCK-8 test results showed that the absorbency (A) values of NC group at 24 and 48 hours were 0. 65 ± 0. 01 and 0. 71 ± 0. 01, which were significantly higher than those of interfering group at 0. 48 ± 0. 07 and 0. 50 ± 0. 08 (P ＜ 0. 05). The number of penetrating cells per field of vision in the interference group at 48 hours was 46 ± 15, which was significantly less than 120 ± 35 in the NC group.Scratch test also confirmed that OVCAR3 cell migration ability decreased significantly after TUG1 interference. Seahorse results showed that the decreased expression of TUG1 significantly reduced mitochondrial respiration and glycolysis flux in OVCAR3 cells. Conclusion High expression of lncRNA TUG1 can promote the proliferation, migration and energy metabolism of ovarian cancer cells.