Expression analysis of microRNA-155-5p in hepatocellular carcinoma tissues and its clinical significance

WU Yunlong; SHI Heping; ZHU Yidong

doi:10.3969/j.issn.1009-0460.2018.12.007

Abstract：Objective To investigate the level of microRNA-155-5p (miR-155-5p) in hepatocellular carcinoma (HCC) tissues and its relationship with clinicopathological parameters and prognosis.Methods A total of 114 pairs of HCC tissues and paracancerous tissues were collected from January 2013 to December 2015.The levels of miR-155-5p in the above tissues were detected by real-time fluorescence quantitative PCR (QPCR). The relationships between miR-155-5p levels and the clinicopathological parameters of HCC (sex, age, number of tumor, tumor size, degree of differentiation, tumor capsule, TNM stage, Child-Pugh grade and alpha-fetoprotein level) were analyzed.According to the follow-up data, the relationship between different levels of miR-155-5p and prognosis of HCC patients was analyzed.Results The results of QPCR showed that the level of miR-155-5p in HCC tissues was 3.041±1.393, which was higher than 1.167±0.514 in paracancerous tissues (P＜0.05). The level of miR-155-5p was correlated with the number of tumor, tumor size, degree of differentiation and TNM stage in HCC patients (P＜0.05), but not with sex, age, tumor capsule, ChildPugh grade and alpha-fetoprotein level (P＞0.05). HCC patients were followed up for 8 to 60 months, with a median follow-up time of36.0 months and a median overall survival (OS) of 46.0 months.The median OS of 60 cases with high-expression of miR-155-5p was37.0 months, and that of 54 cases with low-expression was 57.0 months.The median OS of low-expression of miR-155-5p was higher than that of high-expression (P＜0.05). Conclusion The up-regulation of miR-155-5p in HCC tissues is involved in the occurrence and development of HCC.The level of miR-155-5p with high tumor load, poor differentiation and late staging is higher, and the prognosis of high miR-155-5p level is worse.This molecule may be a potential prognostic indicator of HCC.