Expression and clinical significance of microRNA-150 in non-small cell lung cancer tissues

XU Jianlin; ZHENG Ruifeng; WANG Yanwei

doi:10.3969/j.issn.1009-0460.2018.12.005

Abstract：ObjectiveTo evaluate the expression and clinical significance of microRNA-150 (miR-150) in non-small cell lung cancer (NSCLC) tissues.Methods Seventy-three pairs of cancerous tissues and matched adjacent tissues from 73 patients with NSCLC who underwent surgical excision from September 2014 to June 2017 were collected.Quantitative real-time PCR (QPCR) was performed to evaluate miR-150 level of the above tissues.The relationship between NSCLC and its clinicopathological features (sex, age, smoking history, tumor size, lymph node metastasis, TNM stage and pathological type) was analyzed.The prognosis of NSCLC patients with different miR-150 levels was analyzed based on follow-up data.ResultsThe level of miR-150 in NSCLC tissues was2.014±0.227, higher than 1.023±0.154 in adjacent tissues (P＜0.05). The expression of miR-150 in NSCLC patients was not related to gender, age, smoking history and pathological type, but related to lymph node metastasis, tumor size and TNM stage (P＜0.05). The miR-150 level was higher in patients with positive lymph node metastasis, larger tumor size and late clinical stage.Kaplan-Meier survival curve analysis showed that the overall survival of patients with high-expression of miR-150 was 27.0 months, which was lower than 34.0 months of patients with low-expression (P＜0.05). Conclusion MiR-150 is highly expressed in NSCLC tissues and participates in the occurrence and development of NSCLC.Those with higher miR-150 level have poor prognosis and miR-150 can be used as potential diagnostic and prognostic monitoring markers of NSCLC.