Expression of lncRNA SNHG1 in papillary thyroid carcinoma and its effect on cell proliferation and apoptosis
LIU Wei
LI Chao
SHUI Chunyan
Abstract：Objective To investigate the expression of long-chain non-coding RNA small nucleolar RNA host gene 1 (SNHG1) in thyroid papillary carcinoma (PTC) tissues and cells and its effect on the proliferation and apoptosis of PTC cells.Methods Quantitative real-time fluorescence PCR (QPCR) was used to detect the expression of SNHG1 in tumors and adjacent tissues of 40 PTC patients undergoing surgical treatment, and the expression of SNHG1 in different PTC cell lines (K1, BCPAP and TPC-1) was also detected.BC-PAP and TPC-1 cells were transfected with si-SNHG1-2 and si-SNHG1-3, and cells transfected with si-NC were used as negative control group.MTT assay was used to detect the changes of cell proliferation.Flow cytometry was used to detect the changes of cell cycle and apoptosis, and Western blotting was used to detect the changes of p53 pathway-related proteins.Results Compared with normal tissues adjacent to cancer, the expression of SNHG1 in PTC tissues increased significantly (2.72±0.97 vs.4.72±1.14, P＜0.05). The expression levels of SNHG1 in K1, BCPAP and TPC-1 cell lines were 2.82±0.25, 5.31±0.51 and 3.92±0.47, all higher than that in human normal thyroid Nthy-ori3-1 cells (P＜0.05). Compared with si-NC group, the proliferative rates of BCPAP cells in si-SNHG1-2 and siSNHG1-3 groups were (50.22±4.65) ％and (58.19±5.46) ％ (P＜0.05), while those of TPC-1 cells in si-SNHG1-2 and si-SNHG1-3groups were (47.18±4.27) ％and (51.24±6.49) ％ (P＜0.05). The G2/M phase ratio of BCPAP and TPC-1 cells in si-NC group was lower than that in si-SNHG1-2 group and si-SNHG1-3 group.The apoptotic rate of BCPAP cells in si-NC group was (10.71±0.78) ％, lower than (24.90±1.87) ％and (26.89±1.44) ％in si-SNHG1-2 group and si-SNHG1-3 group (P＜0.05). The apoptotic rate of TPC-1cells in si-NC group was (11.12±1.05) ％, lower than (27.21±2.09) ％and (29.67±2.14) ％in si-SNHG1-2 group and si-SNHG1-3group (P＜0.05). Silencing the expression of SNHG1 could decrease the expression of MDM2 and increase the expression of p53 and p21.Conclusion SNHG1 expression is up-regulated in PTC tissues and cells.Silencing SNHG1 expression may inhibit cell proliferation, affect cell cycle change and promote apoptosis by activating p53 pathway.
Keywords：Papillary thyroid carcinoma (PTC)Small nucleolar RNA host gene 1 (SNHG1)ProliferationCell cycleApoptosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1057-1062 )