ADAM15 promotes metastasis and invasion of colorectal cancer cells by activating EGFR

WANG Xinwei; LV Ke; SONG Zhan; QIAN Guowu; YU Sijun

doi:10.3969/j.issn.1009-0460.2018.10.004

Abstract：Objective To investigate the expression of ADAM15 in colorectal cancer cells and its effect on migration and in-vasion of colorectal cancer cells. Methods The expression of ADAM15 protein in human normal colorectal mucosal cell line FHC and human colorectal cancer cell line Caco-2,SW-480 and DKO-1 was detected by Western blotting. SW-480 cells were transfected into siNC (siNC group)and siADAM15 (siADAM15 group)respectively. Cell scratch test and Transwell test were used to detect the mi-gration and invasion ability of the two groups.The expressions of p-EGFR,EGFR and ADAM15 proteins in siNC,siADAM15 and FHC were detected Western blotting. Results The expression levels of ADAM15 in Caco-2,SW-480 and DKO-1 cell lines were 2.01 ± 0.149,2.74 ±0.053 and 2.27 ±0.185,respectively,which were higher than 0.98±0.043 in FHC cell line,and the difference was statistically significant (P＜0.05). Scratch test showed that the ratio of 24 h scratch width to 0 h scratch width in siADAM15 group was 0.925±0.087,which was significantly higher than 0.326±0.031 in siNC group,and the difference was statistically significant (P＜0.05). Transwell test showed that the invasive number of SW-480 cells in siADAM15 group was 327.4±2.13,which was significantly lower than 669.7±35.21 in siNC group (P＜0.05).The expression of p-EGFR in SW-480 cells of siNC group was 0.89±0.097,which was significantly higher than 0.42 ±0.035 in FHC cell line,and the difference was statistically significant (P＜0.05). The expression of p-EGFR in SW-480 cells of siADAM15 group was 0.27±0.067,which was significantly lower than 0.89±0.097 of siNC group,and the difference was statistically significant (P＜0.05). Conclusion ADAM15 may promote the migration and invasion of colorectal cancer cells by activating EGFR.