Effect of Fasudil on proliferation,invasion and metastasis of gastric cancer MGC-803 cells

WANG Jun; MA Xing

doi:10.3969/j.issn.1009-0460.2018.10.003

Abstract：Objective To investigate the effect of Fasudil on the pfoliferation,invasion and migration of gastric cancer MGC-803 cells and their probable mechanisms. Methods MGC-803 cells were treated with different concentration of Fasudil and cell num-ber was counted. And the subsequent experiments were conducted in 3 groups respectively contained 10,25,50 μmol/L of Fasudil, the MGC-803 cells treated with Fasudil were taken as the treatment group,while those not treated with Fasudil were taken as the control group. The cells were cultured for 4 days(0,24,48,72,96 h),and the cell growth folds were detected by CCK-8.The ability of cell migration was detected by wound-healing assay. The ability of cell invasion was detected by Transwell. The protein expression levels of vascular endothelial growth factor (VEGF),matrix metalloproteinases 9(MMP-9),matrix metalloproteinases14 (MMP-14)and epi-thelial mesenchymal transformation (EMT)markers Vimentin and E-cadherin were detected by Western blotting. Results The toxicity test showed that Fasudil inhibited the cell growth of MGC-803 cells in a dose-dependent manner and the cell growth folds were reduced in the treatment group. There was an obvious decrease in the growth folds compared to the control group (P＜0.01). The results of wound healing assay showed that the wound closure rate in the treatment group was lower than that of the control group,and in a dose-dependent manner(P＜0.01). Transwell results showed that the number of invasive cells in the treatment group was decreased signifi-cantly compared with the control group (P＜0.01). Besides,Fasudil significantly decreased the expression levels of VEGF,MMP-9, MMP-14 and Vimentin,and up-regulated the expression levels of E-cadherin. Conclusion Fasudil may inhibit cell proliferation,mi-gration and invasion of MGC-803 cells,reduce the activity of MMP-9,MMP-14 and VEGF,which will provide new sight and theoreti-cal basis for the treatment of gastric cancer.