Effects of miR-1253 on proliferation,migration and invasion of lung adenocarcinoma cells
ZHANG Chao
NIU Yi
ZHANG Jie
YAO Xuemin
GAO Peng
LIU Meiyue
WEI Xiaomei
YANG Zhao
LIU Chunling
ZHANG Yueheng
LI Hongmin
SUN Guogui
Abstract：Objective To investigate the expression of microRNA-1253(miR-1253)in lung adenocarcinoma cell lines and its effect on the proliferation,migration and invasion of lung adenocarcinoma cells. Methods Real-time fluorescence quantitative PCR (QPCR)was used to detect the expression of miR-1253 in lung adenocarcinoma cell lines A549,NCI-H1299,NCI-H157,A973 and GLC-82. miR-1253 mimics and miR-1253 inhibitor were transfected into A973 and NCI-H157 cells respectively. The cells transfected with negative control plasmid NC were used as negative control(NC)group. The effects of different expression of miR-1253 on prolifer-ation,clone formation,invasion and migration of A973 and NCI-H157 cells were examined by cell proliferation assay,clone formation assay and Transwell migration and invasion assay. Results The results of QPCR showed that the relative expression of miR-1253 in A549,NCI-H1299,NCI-H157,A973 and GLC-82 cells were 0.92±0.06,0.06±0.03,1.10±0.26,0.03±0.01 and 0.45±0.08, respectively. After transfection of miR-1253 mimics into A973 cells,the relative expression of miR-1253 increased significantly(P＜0.05),and decreased significantly after transfection of miR-1253 inhibitor into NCI-H157 cells (P＜0.05). Compared with the NC group,the transfection of miR-1253 mimics could significantly inhibit the proliferation activity,the ability of plate cloning(166.0 ± 29.3 vs. 371.0±31.4,P＝0.001)and the ability of migration(91.1±32.1 vs.166.7±33.9,P＝0.008)and invasion(74.4±20.5 vs. 145.6±28.8,P＝0.001)of lung adenocarcinoma cell line A973;miR-1253 inhibitor can up-regulate the proliferation,number of plate cloning cells(545.0±61.9 vs. 337.0±39.7,P＝0.008),migration(246.7±36.7 vs. 151.1±32.9,P＜0.001)and invasion(231.1± 38.8 vs. 137.8±27.3,P＝0.001)of NCI-H157. Conclusion miR-1253 could reduce malignant phenotype of lung adenocarcinoma cells,and therefore it can be used as an effective target for gene therapy of lung adenocarcinoma.
Keywords：Lung adenocarcinomamicroRNAsInvasion and metastasisGene therapy
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 865-869 )