Effects of SEPT6 silence on cell proliferation, apoptosis of bladder cancer EJ cells and underlying mechanism
LUO Yu
LIU Feng
YAN Chunhui
ZHU Liang
GUO Zheng
ZHOU Fan
ZHANG Wei
QU Wei
ZHANG Hongyu
ZHAO Changlin
WANG Xiaopu
Abstract：Objective To explore the effects of SEPT6 silence on biological characteristics of bladder cancer EJ cells as well as the underlying mechanism. Methods EJ cells transfected with specific small interfering RNA sequence were used as SEPT6 silence group, and cells transfected with scramble RNA sequence were used as control group. The effects of SEPT6 silence on cell proliferation were detected by CCK-8 assay and cell cycle distribution was evaluated by flow cytometry. The effects on cell apoptosis were detected by Hoechst 33342 staining assay. The phosphorylation of Akt and PTEN protein were evaluated by Western blotting assay. Results In SEPT6 silence group, SEPT6 mRNA level was(23.4±2.3)％, while that in control group was(100.0±2.2)％. After transfection for 72 h, the light absorption value was 2.3±0. 1 in SEPT6 silence group, while that in control group was 3.7±0.4. After transfection for 48 h, the percentage of cells in G0/Gl phase was (64.5±2.8)％ by flow cytometry in SEPT6 silence group, while that in control group was (43.3±4.2) ％. The percentage of apoptosis cells in SEPT6 silence group was (24.2±3.2) ％ and that in control group was (8.2±1.4) ％ by Hoechst 33342 staining assay. The phosphorylation of Akt was decreased while PTEN expression increased after SEPT6 silence. Conclusion SEPT6 silence can inhibit the proliferation of bladder cancer EJ cell and lead to the increase of apoptosis. PTEN/ Akt signal pathway may involve in this process.
Keywords：Bladder cancerSEPT6PTEN/Akt signal pathway
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 211-215 )