Effect of gambogic acid granules on proliferation and apoptosis of colon cancer stem cell

OU Zhitao; WEI Fang; CHEN Zhiqian; GUO Jiawei; LUO Duo

doi:10.3969/j.issn.1009-0460.2018.02.004

Abstract：Objective To investigate the effect of gambogic acid(GA) granules on proliferation and apoptosis of colon cancer stem cells. Methods Colon cancer HCT116 cells were cultured in vitro and ALDH1+/CD133+ colon cancer stem cells were isolated from HCT116 cells by immunomagnetic beads sorting technique. Exposure to different concentrations (0,0.5,1,2 mg/ml) of GA granules,the colony formation ability of colon cancer stem cells was detected by colony forming assay,and cell proliferative ability was detected by CCK-8 assay. The apoptosis of the cells was detected by flow cytometry, and Western blotting and QPCR were used to detect the protein and mRNA levels of Sox2 and Oct4 in stem cells. Results ALDH1+/CD133+ colon cancer stem cells were successfully isolated by immunomagnetic beads sorting technique. Cloning formation assay revealed that cell clone formation rate was decreased significantly in a dose-dependent manner after treatment with different concentrations of GA granules. CCK-8 detection showed that GA granules could inhibit the proliferation of colon cancer stem cells in a time-and dose-dependent manner. Flow cytometry results showed that GA granules could induce apoptosis of colon cancer stem cells in a dose-dependent manner. Western blotting and QPCR results showed that GA granules could decrease the protein and mRNA levels of Oct4 and Sox2 in stem cells in a dose-dependent manner. Conclusion GA granules can inhibit colon cancer stem cell proliferation,cloning,expression of Sox2 and Oct4,and induce cell apoptosis.