Effects of aspirin combined with trastuzumab on proliferation and apoptosis of HER-2 positive breast cancer cells
ZHAO Qingli
MA Ji
Abstract：Objective To investigate the effects of aspirin combined with trastuzumab on proliferation and apoptosis of HER-2-positive breast cancer cell line SKBR-3.Methods The SKBR-3 cells were divided into four groups:control group,aspirin group (5 mmol/L),trastuzumab group (30 μg/ml) and combination group (5 mmol/L aspirin+30 μg/ml trastuzumab).MTT assay and flow cytometry were used to observe the effects of different drug treatment on cell proliferation and apoptosis.Results MTT assays showed that the relative proliferation rate of SKBR-3 cells was (79.6±2.61) ％ in aspirin group,(48.2±3.35) ％ in trastuzumab group and (21.5± 1.66)％ in combination group.Compared with aspirin group or trastuzumab group,the cell proliferation of combination group was significantly inhibited (P＜0.05).Two drugs combined effect index was 1.0 of a superimposition effect.The apoptosis assays showed that the relative apoptotic rate of cells was (27.3±3.47)％ in aspirin group,(35.3±t2.80)％ in trastuzumab group and (56.2 ±3.95)％ in combination group.Compared with aspirin group or trastuzumab group,the cell apoptosis of combination group was significantly increased (P＜0.05).Conclusion Aspirin combined with trastuzumab can effectively inhibit the growth of HER-2-positive breast cancer cells,which may be a new model for the clinical treatment of HER-2-positive breast cancer.
Keywords：Breast cancerEpidermal growth factor receptor-2 (HER-2)AspirinTrastuzumabProliferationApoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1091-1095 )