Expression and effect of miR-769-5p on proliferation, migration and invasion in non-small cell lung cancer
GAO Peng
YANG Zhao
ZHANG Jie
ZHANG Chao
WANG Wei
ZHAI Hongfang
CHEN Mingda
ZUO Fang
WANG Yan
DU Jiayi
ZHANG Lixin
LI Hongmin
SUN Guogui
Abstract：Objective To explore the expression of miRNA-769-5p (miR-769-5p) in non-small cell lung cancer(NSCLC) and its effect on proliferation,migration and invasion of NSCLC cells.Methods The miR-769-5p expression in NSCLC cell lines (A549,NCI-H1299,NCI-H157,ANIP-973 and GLC-82) was detected by QRT-PCR,and the cell lines of highest and lowest expression of miR-769-5p were applied for the further study.NSCLC cell lines were divided into transfection group and control group,and respectively transfected with miR-769-5p mimics/inhibitor and miRNA mimics control/inhibitor control.Furthermore,cell proliferation,colony formation migration and invasion were respectively analyzed by MST,colony formation,and transwell chamber experiments.Results The expression of miR-769-5p in A549,NCI-H1299,NCI-H157,ANIP-973 and GLC-82 were 0.06,0.40,0.09,1.04 and 2.31.miR-769-5p mimics and miR-769-5p inhibitor were respectively transfected into A549 and GLC-82 cell lines.MST test showed that transfection with miR-769-5p mimics in A549 cells for 48,72,96 h significantly inhibited the cell viability (P＜0.05).The colony formation (124.7±14.7 vs.399.4±46.0,P＜0.05),migration (94.4±18.1 vs.157.8±24.8,P＜0.05) and invasion (84.4±15.1 vs.135.6±16.7,P＜0.05) ability of A549 cell were also suppressed compared with control group after transfection.Down-regulation of miR-769-5p by transfected with miR-769-5p inhibitor could enhance the malignant phenotype of GLC-82 cells in vitro,including cell growth (P＜0.05),colony formation (555.7±29.7 vs.366.3±28.7,P＜0.05),as well as cell migration (226.7±40.3 vs.153.3± 38.7,P＜0.05) and invasion (196.7±39.1 vs.138.9±40.4,P＜0.05).Conclusion The expression of miR-769-5p can inhibit the proliferation,migration and invasion of NSCLC cells.
Keywords：Non-small cell lung cancer (NSCLC)MicroRNA-769-5p(miR-769-5p)InvasionMigration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 769-774 )