Inhibitory effect of NRP-1mAb on the growth of gastric cancer cell BGC-823
DING Yuan
ZHOU Juan
CHEN Yuqiang
YAN Jianghua
PENG Lizhen
Abstract：Objective To observe the effect of NRP-1b1/b2 IgG monoclonal antibody (NRP-1mAb) on the growth of gastric cancer cell BGC-823,and to explore the possible mechanism of the antibody.Methods NRP-1mAb was prepared in laboratory,and the purity of antibody was detected by SDS-PAGE.Gastric cancer BGC-823 cells were cultured by 0,25,100,200,400 μg/ml NRP-1 mAb.The morphological changes of BGC-823 cells were observed by microscope.The proliferation,colony formation and apoptosis of gastric cancer BGC-823 cells were observed by MTT assay,colony forming assay and flow cytometry.The phosphorylation of related signal proteins was detected by Western blotting analysis.Results SDS-PAGE test showed that the purity of NRP-1mAb was above 95％.Microscopy showed apoptotic changes of BGC-823 cells treated by NRP-1mAb.MTT assay showed that NRP-1mAb could inhibit the proliferation of BGC-823 cells in a time and dose dependent manner(P＜0.01).Colony forming assay showed that different doses of NRP-1mAb could inhibit the colony formation of BGC-823 cells in a dose dependent manner(P＜0.01).Flow cytometry showed that different doses of NRP-1mAb could promote the apoptosis of BGC-823 cells mainly at early apoptosis stage.It was found that the level of Akt phosphorylation was decreased after treated by NRP-1mAb,and therc was no significant phosphorylation of ERK,p38 and JNK protein.Conclusion NRP-1mAb can inhibit the growth of gastric cancer cell BGC-823 and promote apoptosis,which may be related to the inhibition of Akt phosphorylation.
Keywords：Gastric cancerNRP-1mAbProliferationProtein phosphorylation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 577-582 )