Impact of Livin antisense oligonucleotide on the activity of osteosarcoma MG-63 cells
LI Chengjun
LIU Xiaozhou
SHI Xin
SUN Chang
ZHANG Lei
WU Sujia
Abstract：Objective To investigate the expression of Livin in osteosarcoma MG-63 cells and the effect of Livin anti-sense oligonucleotide(ASODN) on its activity and function.Methods The ASODN targeting Livin were designed and constructed.According to the results of transfection efficiency evaluation, the liposome/oligonucleotide complexes were prepared by adding oligonucleotide and then transferred into MG-63 cells.The immunohistochemical staining and Western blotting were used to detect the expression of Livin at 24th h after transfection in MG-63 cells.The proliferation at 72nd h and apoptosis at 24th h after transfection were measured by CellTiter-Glo and Flow cytometry,respectively.Results The Livin was mainly expressed in the cytoplasm of MG-63 cells.We chose ASODN with the highest transfection efficiency to transfer MG-63 cells.The expression of Livin protein decreased significantly after the transfection, and Livin ASODN at different concentrations inhibit the proliferation and migration of MG-63 cells with significant difference.The apoptotic rate of Lip-ASODN group was higher than those of blank control group and negative control group in a dose-dependent manner (P<0.05).Conclusion The ASODN targeting Livin can inhibit the proliferative capacity and promote the apoptosis of osteosarcoma MG-63 cells.
Keywords：OsteosarcomaAntisense oligonucleotidesLivin proteinCell activity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 493-499 )