The effect of microRNA-133b on migration and invasion of glioblastoma
ZHANG Dongzhi
CHANG Liang
SU Jun
WANG Chao
TAN Chunlei
LI Guofu
ZHANG Xuexin
Abstract：Objective To investigate the effect of microRNA-133b (miR-133b) on glioblastoma (GBM) cell and to analyze its possible molecular mechanism.Methods Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect the expression of miR-133b in 20 human GBM samples and normal human glial cells HBE.Transwell migration and invasion assays were used to evaluate the effects of miR-133b on cell migration and invasion.Western blotting and a luciferase reporter assay were used to identify the target genes of miR-133b.Results Compared with the HBE, miR-133b was significantly increased in GBM tissues (1.0±0.17 vs.2.42±0.69, P<0.05);In Transwell invasion and migration experiments, compared to group transfected with NC, the cells transfected with miR-133b mimic could inhibit the migration and invasion (P<0.05);In Western blotting, compared to cells that transfected with NC, the cells transfected with miR-133b mimic had a lower expression of MMP-14 protein (P<0.05).Furthermore, MMP-14 was identified as a direct target gene of the miR-133b by luciferase reporter gene analysis.Conclusion miR-133b can inhibit the migration and invasion of GBM by direct targeting MMP-14, and it can be used as a candidate target for diagnosis and treatment of GBM.
Keywords：Glioblastoma(GBM)MicroRNA-133bMatrix metalloproteinase 14(MMP-14)MigrationInvasion
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 391-394 )