Analysis of miR-449a/b/c expressions and their clinical significance in non-small cell lung cancer

HE Wencai; JIN Lishan; LI Hua

doi:10.3969/j.issn.1009-0460.2017.04.003

Abstract：Objective To investigate the expression and clinical significance of miR-449a/b/c in non-small cell lung cancer (NSCLC) as well as their expressions with prognosis.Methods We collected 82 cases of surgically resected NSCLC tissues and 69 cases of adjacent normal tissue (>5 cm away from cancer tissue) from January 2013 to December 2015.The expressions of miR-449a/b/c of above tissues were detected by using real-time fluorescence quantitative PCR (QPCR).The differences of distribution of miR-449a/b/c expressions in NSCLC and adjacent normal tissues were compared.Pearson correlation analysis of miR-449a, miR-449b and miR-449c in NSCLC tissues was undergone.The relationship of miR-449a/b/c expressions in NSCLC with clinical pathological parameters (gender, age, tumor size, TNM stage, histological type and lymph node metastasis) were analyzed.According to the follow-up data, the prognosis of different expressions of miR-449a/b/c was compared.Results QPCR results showed that in NSCLC tissues, the avarage expressions of miR-449a, miR-449b and miR-449c were 0.210±0.028, 0.359±0.031 and 0.133±0.020, lower than those in adjacent normal tissues, and the differences were statistically significant (P<0.05).The expressions of miR-449a, miR-449b and miR-449c were positively correlated in NSCLC and correlation coefficients for miR-449a/b, miR-449a/c and miR-449b/c were 0.246, 0.390 and 0.331 (P<0.05).The miR-449a/b/c levels of NSCLC tissues were significantly associated with TNM stage, and miR-449a and miR-449c were related to tumor size.All patients were followed up, and the median overall survival (OS) was 12.4 months.The median OS for miR-449a low expression group and high expression group were 11.2 and 13.5 months (P>0.05).The median OS for miR-449b low expression group and high expression group were 9.6 and 14.2 months (P<0.05).The median OS for miR-449c low expression group and high expression group were 11.7 and 13.7 months (P>0.05).Conclusion The expression of miR-449a/b/c in NSCLC tissues was decreased, and all of them were related to TNM stage.The expression of miR-449b was related to prognosis, which may be related to the occurrence and development of NSCLC.It has some value in the diagnosis and evaluation of NSCLC.