Effects of miR-138 on cell proliferation and apoptosis of bladder cancer via targeting SIRT1
HUANG Zhuoya
MIAO Weixian
WANG Xiaobing
ZHU Shuling
WU Tongtong
Abstract：Objective To investigate the expression of microRNA-138(miR-138) in bladder cancer T24 cells and to study its effect on proliferation and apoptosis of bladder cancer cells as well as its possible target genes.Methods Real-time quantitative PCR (QPCR) was used to detect the expression of miR-138 in bladder cancer cell line T24 and normal bladder epithelial cells SV-HUC-1.T24 cells were divided into normal control group, miR-138 negative control group and miR-138 transfection group, which were transfected with none, negative control fragment and miR-138 mimics.MTT assay and flow cytometry were performed to determine the effect of miR-138 on cell proliferation and apoptosis.Western blotting was used to detect silent mating type information regulation 2 homolog 1 (SIRT1) protein levels in each group.Dual luciferase reporter assay was used to confirm the target relationship between miR-138 and SIRT1.Results The level of miR-138 in bladder cancer T24 cells was 0.57±0.19, lower than 1.00±0.26 of normal bladder epithelial cells, and the difference was statistically significant (P<0.05).The miR-138 level of miR-138 transfection group was 2.59±0.67, higher than 1.00±0.36 of normal control group and 1.08±0.49 of miR-138 negative control group (P<0.05).The proliferation rate of miR-138 transfection group was lower than that in miR-138 negative control group and normal control group with statistical significance (P<0.05).Forty-eight hours after transfection, the apoptotic rate of miR-138 transfection group was (29.8±1.9)%, higher than (5.8±1.2)% of normal control group and (7.7±0.9)% of miR-138 negative control group (P<0.05).The relative expression of miR-138 in miR-138 transfection group was 0.59±0.22, lower than 1.00±0.35 of normal control group and 1.20±0.42 of miR-138 negative control group (P<0.05).The double luciferase reporter assay further confirmed that SIRT1 was a direct target of miR-138.Conclusion MiR-138 is decreased in bladder cancer cells, and the over-expression of miR-138 may inhibit the proliferation and promote apoptosis of bladder cancer cells by targeting SIRT1.
Keywords：Bladder cancerMicroRNA-138(miRNA-138)Silent mating type information regulation 2 homolog 1 (SIRT1)ProliferationApoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 298-302 )