Relationship between serum miR-143 level and the sensitivity of paclitaxel-based chemotherapy regimen in advanced gastric cancer

LI Jinfeng; CHENG Weili; REN Xusheng; LI Xianghong; LI Dapeng

doi:10.3969/j.issn.1009-0460.2017.02.008

Abstract：Objective To investigate the relationship between serum microRNA-143 ( miR-143) level and chemosensitivity to paclitaxel in patients with advanced gastric cancer. Methods A total of 82 patients with advanced gastric cancer treated with paclitax-el-based regimen in our hospital from January 2012 to July 2016 were enrolled. Serum samples were collected before chemotherapy and their miR-143 levels were detected by qPCR method, and then compared with 76 healthy persons in the same period of miR-143. The relationship between different miR-143 levels and clinical pathological characters were analyzed. RECIST1. 1 standard was used to eval-uate the short-term efficacy and the relationship between different levels of miR-143 and chemosensitivity were evaluated. Median pro-gression free disease ( PFS) and survival ( OS) were compared between different miR-143 levels according to follow-up data. Results The qPCR detection showed that the miR-143 level of 82 patients with gastric cancer was (0. 215±0. 021), lower than those of the healthy subjects (1. 099±0. 057), and the difference was statistically significant (P<0. 05). Serum miR-143 level was not related to sex, age, ECOG score, tumor location and pathological type, but with TNM stage and differentiation degree, and the difference was statistically significant (P<0. 05). The serum miR-143 level of 31 patients with chemotherapy sensitive (CR+PR) was (0. 341± 0. 040), which was higher than that of chemotherapy resistance (SD+PD) in patients (0. 138± 0. 015), and the difference was statis-tically significant (P<0. 05). The patients were divided into high expression group (>0. 177) and low expression group (≤0. 177) according to the medium value. The response rate, median PFS and OS in the high expression group were 48. 8%, 8. 5 and 12. 2 months, significantly better than those in the low expression group (31. 7%, 7. 3 and 9. 4 months), and the difference was statistically significant ( P<0. 05) . Conclusion The serum miR-143 level of advanced gastric cancer is lower than normal, and related to the pa-clitaxel chemotherapy sensitivity and prognosis. High expression of miR-143 had a higher sensitivity and better prognosis, showing a certain value in the diagnosis and prognosis of gastric cancer.