Study on the mechanism of sodium valproate combined with G-CSF induced myeloid leukemia cell differen-tiation
ZHAO Shuqing
LI Junmin
XU Zizhen
YHANG Chenmin
Abstract：Objective To explore the mechanism of sodium valproate ( VPA) combined with granulocyte colony-stimulating factor( G-CSF) in the induction of differentiation of U937 leukemic cell line. Methods The U937 cells at logarithmic growth phase were treated with 0. 5 mmol/L VPA or 50 ng/ml G-CSF alone and in combination. Cell differentiation was analyzed by Wright-Giemsa stain cell morphology and flow cytometry. Flow cytometry was performed to detect the expression of G-CSFR in the cells. The protein level of CCAAT/enhancer-binding proteins C/EBPα and G-CSFR were detected by Western blotting. Results VPA induced myeloid differentiation in U937 cells as demonstrated by up-regulation of CD11b from(11. 49±3. 48)% to (62. 48±9. 96)% (P<0. 05). When combined with G-CSF, the differentiation was remarkably enhanced to(97. 65±0. 49)% (P<0. 05). The expressions of G-CSFR (CD114) on U937 cells were also up-regulated from (29. 50±6. 80)% to (59. 88±9. 99)% (P<0. 05). VPA can enhance C/EBPαexpression in these cells. The expression levels of G-CSFR protein were significantly up-regulated. Conclusion Combinations of VPA with G-CSF had an additive or synergistic effect on induction of myeloid differentiationin U937 cells. This effect may be mainly through the increase of C/EBPα and up-regulation of G-CSFR.
Keywords：Acute myeloid leukemiaSodium valproateGranulocyte colony-stimulating factor( G-CSF)Cell differen-tiation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 114-117 )