Silencing effect of TRIM29 on proliferation, apoptosis and invasion of osteosarcoma cells
NIE Yu
ZHOU Naxin
Abstract：Objective To investigate the effect of small interfering RNA (siRNA) targeting tripartite motif-containing 29 (TRIM29) on the proliferation,apoptosis and invasion of osteosarcoma cells.Methods The levels of TRIM29 in osteosarcoma cell lines (MG63 and U2OS) were detected by fluorescence real-time quantitative PCR (QPCR) assay.Two siRNA fragments (siTRIM29-1 and siTRIM29-2) were efficiently transfected into MG63 and U2OS cells by lipofectamine method,and MG63 and U2OS cells transfected with siControl were set as control.The highest inhibitive efficiency of siRNA was selected for subsequent functional study.The proliferation,apoptosis and invasion of MG63 and U2OS cells after transfection were evaluated by MTT,flow cytometry and Transwell assay.Results The results of QPCR test showed that TRIM29 mRNA of MG63 and U2OS cells were significantly higher than those of normal osteoblast cell line hFOB1.19 (P＜0.05).Transfection of TRIM29 siRNA could reduce the TRIM29 level of MG63 and U2OS cells at 72 h.Compared with the cells transfected with siControl,there were decreased survival rates and transmembrane cell number,but increased apoptotic rates in transfection group with statistical significance (P＜0.05).Conclusion TRIM29 is highly expressed in osteosarcoma cells,and silencing of TRIM29 can inhibit the proliferation and invasion of osteosarcoma cells and induce cell apoptosis,which can be used as reference in the treatment of osteosarcoma.
Keywords：OsteosarcomaSmall interfering RNA (siRNA)Tripartite motif-containing 29 (TRIM29)ProliferationApoptosisInvasion
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1079-1084 )