Screening of differentially expressed Wnt signaling pathway genes in hepatocellular carcinoma and its sig-nificance
YE Yingquan
ZHU Zhongzheng
WANG Liangliang
BAO Lingling
CONG Wenming
WEN Bingji
Abstract：Objective To detect the differentially expressed Wnt signaling pathway genes in hepatocellular carcinoma ( HCC) , and investigate the relationship between HCC and Wnt signaling pathway. Methods The expression levels of 78 genes relat-ed Wnt signaling pathway were measured using Affymetrix GeneChip Human Genome U133 Plus 2. 0 array in 93 cases of HCC tissues and paired non-tumor liver tissues. Results A total of 10 up-regulated genes ( TCF19, MMP-12, DKK1, Bcl-9, SFRP4, MMP-1, PYGO2, FZD6, MMP-9 and Wnt6) , and 5 down-regulated genes ( SFRP1, TCF21, SFRP5, FZD1 and Wnt2) were identified to be significantly differentially expressed in HCC tissues, compared with paired non-tumor liver tissues. The expression level of TCF19, the most over-expressed gene in this series (fold change=9. 61, P=1. 07×10-26), was significantly correlated with FZD1, FZD6, Bcl-9, Wnt6, MMP-1 and MMP-12 ( P<0. 05) . The expression of TCF19 was significantly associated with the number of tumor ( P=0. 017) and Edmondson-Steiner grade ( P=0. 046) . Conclusion Multiple differentially expressed genes in Wnt signaling pathway in HCC are screened. Increased expression of TCF19, a downstream transcription factor, may be the key terminal effect of the Wnt signaling path-way activation, and its biological and pathological significance in HCC merits further investigations.
Keywords：Hepatocellular carcinomaWnt signaling pathwayDifferential expression geneOligonucleotide microarray
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 980-984 )