Methods and criterion of fluorescence in situ hybridization for detecting pancreatobiliary tract malignancy
PU Xiaohong
YE Qing
CHEN Jun
BO Tao
ZHENG Jinyu
Abstract：Endoscopic retrograde cholangiocretography ( ERCP ) and routine cytology are general approaches for pre?oprative dignosis of suspected pancreatic duct cancer patients. Routine cytology is with high specificity and low sensitivity. The detection of ane?uploidy of 3/7/17 and loss of p16 by fluorescence in situ hybridization( FISH) has revolutionized how laboratories diagnose cholangio?carcinoma and pancreatic adenocarcinoma with increasing sensitivity and minimal decreases in clinical specificity. Although using FISH for diagnostic aim has been more than ten years in foreign, it has not applied to clinical diagnosis in China. Our hospital has promoted this technique and published an article in Chinese Journal of Pathology. However, many peers in other hospitals wonder detailed meth?ods and criterion of this technique, so we provide the information to detect pancreatobiliary cancers in FISH.
Keywords：Cholangiocarcinoma and pancreatic adenocarcinomaFluorescence in situ hybridization( FISH)Cytology
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 854-857 )