Expression and clinical significance of long non-coding RNA CCAT1 in hepatocellular carcinoma tissues and cell lines
SUN Weipeng
NIE Changfu
HAN Feng
ZHANG Chi
QIU Dapeng
ZHANG Xianzhou
Abstract：Objective To explore the expression of long non-coding RNA of colon cancer associated transcript 1 ( LncRNA-CCAT1) in hepatocellular carcinoma( HCC) tissues and cell lines, as well as its clinical significance. Methods CCAT1 expression in 42 pairs of HCC tissues and paired adjacent normal tissues as well as 4 types of HCC cell lines ( SMMC-7721, Hep3B, Hub7 and HepG2) and normal hepatic cell line LO2 were tested by QPCR. The relationship between the expression of CCAT1 and common clini-cal pathological parameters ( gender, age, alpha-fetoprotein, tumor size, tumor number, lymph node metastasis, differentiation and TNM stage) were analyzed accordingly. Results Compared with normal hepatic cell line LO2, there was a high expression of CCAT1 in SMMC-7721, Hep3B, Hub7 and HepG2 cell lines with significant difference ( P<0. 05) , especially in HepG2 cell line. The expres-sion of CCAT1 in HCC tissue was higher than that in the adjacent tissues, and the difference was statistically significant ( P<0. 05) . The expression of CCAT1 in HCC was related with tumor size, alpha fetoprotein, differentiation, TNM stage and lymph node metastasis ( P<0. 05) , but not with age, gender and tumor number( P>0. 05) . Conclusion LncRNA CCAT1 has a role in the occurence, devel-opment and prognosis of HCC, worthy of further research on its mechanism.
Keywords：Long non-coding RNA(lncRNA)Colon cancer associated transcript 1(CCAT1)Hepatocellular carcinoma (HCC)
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 325-328 )