Effect of morphine on proliferation of human lung adenocarcinoma A549 cells
TONG Jianhua
TAN Yuanhui
JI Ningning
XIA Ming
DUAN Manlin
XU Jianguo
Abstract：Objective To explore the effects of morphine on the proliferation and apoptosis of A549 cells. Methods The CCK?8 was used to detect the proliferation of A549 cells at 48 h after treatment with different concentrations of morphine ( 0?3, 3, 30μg/ml) . Annexin?V FITC/PI double staining was used to detect the apoptosis rate of 30μg/ml morphine at 48 h. Western blotting was used to detect the expression levels of X?linked apoptosis inhibitory protein (XIAP), survivin, Bcl?2, caspase?3 and Bax of 30μg/ml morphine at 48 h. Results Morphine could dose?dependently stimulate the proliferation of A549 cells at different concentrations, and the differences were statistically significant (P<0?05). Compared with the control group, the apoptotic rate of A549 cells was de?creased after treatment with 30μg/ml morphine ( P<0?05) . The treatment with morphine leaded to the increased levels of Survivin and XIAP but decreased level of caspase?3( P<0?05) . Conclusion Morphine could promote proliferation and inhibit the apoptosis of A549 cells, possibly via the upregulation of XIAP and survivin and inhibition of caspase?3 activation.
Keywords：MorphineLung cancerProliferationApoptosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 204-207 )