Effects of Rho kinase inhibitor Y27632 on proliferation, apoptosis and invasion of non-small cell lung cancer

ZHU Ying; LIU Bona

Abstract：Objective To investigate the effect of Rho kinase inhibitor Y27632 on cell proliferation, apoptosis and invasion and metastasis of non?small cell lung cancer. Methods The non?small cell lung cancer cells A549 were treated with different concen?trations of Y27632 ( 5, 10, 20, 40, 80μmol/L) and the cells without treatment were set as the control group. The absorbance of A549 cells was detected by MTT method at 24, 48, 72 h after stimulation, and the inhibitory rates of proliferation and half inhibitory concen?trations ( IC50 ) were calculated accordingly. The cell apoptotic rates were measured at 24 and 48 h by Annexin?enhanced green fluores?cent protein (Annexin?EGFP)/iodinated propidium (PI) double staining via flow cytometry. The distribution of cell cycle, including G0/G1 , S and G2/M, were detected by PI single staining with flow cytometry. The number of cells penetrating the membrane was de?tected by Transwell test. Results Y27632 can inhibit the proliferation of A549 ( compared with the control group P<0?05) in a dose?and time?dependence manner, and the IC50 value decreased with the prolongation of the time. Compared with the control group, the ap?optotic rates and G0/G1 phase cell proportions were increased, and the proportions of S and G2/M cells were decreased, and the differ?ences among different concentrations were statistically significant ( P<0?05) . Transwell experiments showed that Y27632 could inhibit the invasion of A549 cells. For instance, the number of cells penetrating the membrane were (78?1±6), (62?3±5?7), (51?7±5?2), (42?3±6?9), (36?4±5?3) and (22?8±4?6) for treatment with 0, 5, 10, 20, 40, 80 mol/L Y27632, respectively. Conclusion In addition to the inhibition effect on cell invasion, Y27632 can inhibit the proliferation of A549 cells and induce apoptosis and G0/G1 ar?rest, having a certain value in the treatment of lung cancer.