Expression of ubiquitin ligase Hrd1 and LAT1 in gastric carcinoma and their significance

PENG Junlu; SHI Lianghui

Abstract：Objective To investigate the expressions of ubiquitin ligase Hrd1 and L?type amino acid transporter 1( LAT1) in gastric carcinoma tissues and their clinical significance. Methods The expression of Hrd1 and LAT1 in 62 samples of gastric carcinoma and adjacent normal tissues was detected by immunohistochemical SP method,RT?PCR and Western blotting. Results The positive ex?pression rate of Hrd1, LAT1 protein in gastric carcinoma tissues was 54?8%( 34/62) and 56?4%( 35/62) , higher than 25?0%( 5/20) and 20?0%(4/20)of adjacent normal tissue with significant differences(P<0?05). The expression of Hrd1 in gastric carcinoma tissues was only related with tumor size, and LAT1 expression was related with tumor size and clinical stage(P<0?05). RT?PCR showed that the quantitive expression of Hrd1 and LAT1 were 4?22±0?83 and 5?80±1?72 of grastric carcinoma tissue, higher than 1?14±0?39 and 1?32± 0?77 of adjacent normal tissue(P<0?05). Western blotting showed that the quantitive expression of Hrd1 and LAT1 were 0?855±0?153 and 0?896±0?148, higher than 0?357±0?178 and 0?439±0?136 of adjacent normal tissue(P<0?05). There was a positive correlation be?tween the expression of Hrd1 and LAT1 in gastric carcinoma( r=0?820,P<0?001) . Conclusion Hrd1 and LAT1 are highly expressed in gastric carcinoma. They may play an important role in the occurrence and development of gastric carcinoma.