The relationship between multi-mutation of HBV X gene and hepatocellular carcinoma
YANG Wei
LI Zengcai
NI Xiuying
WANG Tao
Abstract：Objective To investigate the relationship between multi?mutation of HBV X gene and hepatocellular carcinoma (HCC). Methods The live or liver cancer tissuses of 39 patients with HBV infected were divided into HCC group(25 cases) and chronic hepatitis B ( CHB ) group ( 14 cases ) . The HBV X gene were amplificated from above tissues by polymerase chain reaction ( PCR) , and the amplified products were sequenced directly. Furthermore, the mutation of common mutation site of HBV X gene were determined using soft of Bio Edit7?0 and ClustalW by computer. Results In HCC group, there were higher gene mutation frequency of insert/deletion mutation and combined mutation than those in CHB group. There was higher 1762T/G1764A double mutation frequency in HCC group than that in CHB group. The high frequency of mutation in HBV X gene were as follows:C1655T/G, A1605C/G, A1762T, G1764A, A1772B, A1645C, C1687A and G1776T. Conclusion The mutation of the HBV X region including point muta?tion, insertion/deletion mutation and combined mutation, which may play an important role in the occurrence and development of HCC.
Keywords：Hepatoceller carcinomaHepatitis B virus( HBV) X geneMutation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 726-729 )